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Detection of ureaplasma by multiplex PCR.
To develop a simple and rapid method for detection of human ureaplasmas, particularly Mycoplasma hominis. Use of the multiplex PCR assay that had been developed to identify the Mycoplasma hominis genome and ureaplasmas combined with the direct colony and pure culture assays. DNA samples from clinical specimens, including vaginal swabs, amniotic fluid and urine, were used to establish the specificity of the multiplex PCR. The PCR was also applied to detect ureaplasmas in the placenta and oral swabs. The developed multiplex PCR technique had an identical specificity to conventional PCR assays. Only M. hominis and ureaplasmas were detected in all clinical specimens examined by multiplex PCR, while neither Mycoplasma genitalium nor Ureaplasma urealyticum was detected by any PCR assay. The multiplex PCR developed in this study would be useful to detect all ureaplasmas. This is the first report of detection of ureaplasmas by the multiplex PCR method.Q:
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The alar flap advancement technique is an effective procedure to treat preauricular hair loss, occipital sweat gland hyperhidrosis, or scarring. It allows for full coverage of a single earlobe and results in a natural, symmetrical look. The ear reconstruction procedure results in reconstruction of ear tissue and skin.
Alar Flap Advancement
It is a technique used in ear reconstruction to address preauricular alopecia and select occipital sweat gland hyperhidrosis. In the ear reconstruction procedure, the ear flap advancement technique is used to address the alopecia and postoperative scarring.
The goal of the ear reconstruction procedure is to restore symmetry to the skin. The ear reconstruction procedure helps patients achieve a more natural look and feel to the earlobe, taking away the visual discomfort of the abnormal scarring or defect.Pharmacological action of alkylresorcinol derivatives on inflammatory mediators.
Alkylresorcinols (AR) are phytochemicals abundantly present in human diet. Previous studies have shown that AR derivatives from rye grains have anti-inflammatory activity in different models of inflammation. Here, we investigated the effects of two AR derivatives of wheat bran (ARW1 and ARW2) on inflammatory mediators in cultured vascular endothelial cells (EC) and in human neutrophils. Cytokine release induced by lipopolysaccharide (LPS) and interferon gamma (IFN-gamma) was attenuated by ARW1 in EC at a concentration of 100 microm. ARW2 (100 microm) also attenuated the release of tumor necrosis factor (TNF-alpha) in L 0b46394aab